cicCartuja Centro de Investigaciones Científicas de la cartuja
Título: Global identification of regulatory RNAs in the cyanobacterium Nostoc sp. PCC 7120. Functional characterization of Yfr1 and NsiR1
Doctorando: Francisco Rivero Rodríguez (IIQ)
Fecha: Viernes, 17 de enero de 2020. 11:00 h
Lugar: Salón de Actos
Directores de tesis: Dr. D. Miguel Ángel de la Rosa Acosta y la Dra. Dª. Irene Díaz Moreno
Resumen
The maintenance of genome integrity is a critical process for cell life. To face DNA lesions, living beings have developed a complex network called DNA damage response (DDR), which encompasses an intricate phosphorylation signalling cascade, enabling a quick DNA damage repair. To control this signalling cascade and to avoid an illegitimate activation, this network is tightly regulated by protein phosphatases, such as Protein Phosphatase 2A (PP2A). Moreover, histone chaperones assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several histone chaperones. Within this context, the role of nuclear cytochrome c has been partially disclosed.
Here, we have delved into the functional consequences of the novel DNA damage-induced complex formed by cytochrome c with the histone chaperones ANP32A and ANP32B. Strikingly, the heme protein impairs the PP2A inhibitory effect exerted by the histone chaperones ANP32A, ANP32B and SET/TAF-I. Furthermore, biophysical and structural approaches have been used to characterize the cytochrome c complex with ANP32A and ANP32B and to unveil the molecular mechanism underlying the cytoplasmic accumulation of ANP32B upon its phosphorylation.
In addition, we have extended our knowledge on the histone chaperone regulation in the DDR by studying the Arabidopsis thaliana complex between cytochrome c and NRP1, a SET/TAF-I analogous.
Herein we propose a novel standpoint about DDR regulation, in which cytochrome c exerts a modulating role on such signalling cascade through its interaction with histone chaperones, both by tuning their nucleosome assembly and PP2A inhibitory activity.
Coordinado por el Grupo de Nanotecnología en Superficies y Plasma, del ICMS.
El ganador es José Luís Santos del Instituto de Ciencia de los Materiales (ICMS).
Nuestras investigadoras de los tres institutos, promueven y divulgan ciencia.
Mantuvo un encuentro con nuestros jóvenes postdocs y visitó las instalaciones.
El investigador del IIQ, ha obtenido este reconocimiento de la prestigiosa revista "Chemistry A European Journal"
La Sociedad Española de Biofísica ha seleccionado como artículo de Julio el trabajo de Gonzalo Pérez Mejías, del IIQ.
El 9 de octubre se celebra el NanoDay, donde el Instituto de Ciencia de los Materiales de Sevilla participa.
