Título: Global identification of regulatory RNAs in the cyanobacterium Nostoc sp. PCC 7120. Functional characterization of Yfr1 and NsiR1
Doctorando: Francisco Rivero Rodríguez (IIQ)
Fecha: Viernes, 17 de enero de 2020. 11:00 h
Lugar: Salón de Actos
Directores de tesis: Dr. D. Miguel Ángel de la Rosa Acosta y la Dra. Dª. Irene Díaz Moreno
Resumen
The maintenance of genome integrity is a critical process for cell life. To face DNA lesions, living beings have developed a complex network called DNA damage response (DDR), which encompasses an intricate phosphorylation signalling cascade, enabling a quick DNA damage repair. To control this signalling cascade and to avoid an illegitimate activation, this network is tightly regulated by protein phosphatases, such as Protein Phosphatase 2A (PP2A). Moreover, histone chaperones assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several histone chaperones. Within this context, the role of nuclear cytochrome c has been partially disclosed.
Here, we have delved into the functional consequences of the novel DNA damage-induced complex formed by cytochrome c with the histone chaperones ANP32A and ANP32B. Strikingly, the heme protein impairs the PP2A inhibitory effect exerted by the histone chaperones ANP32A, ANP32B and SET/TAF-I. Furthermore, biophysical and structural approaches have been used to characterize the cytochrome c complex with ANP32A and ANP32B and to unveil the molecular mechanism underlying the cytoplasmic accumulation of ANP32B upon its phosphorylation.
In addition, we have extended our knowledge on the histone chaperone regulation in the DDR by studying the Arabidopsis thaliana complex between cytochrome c and NRP1, a SET/TAF-I analogous.
Herein we propose a novel standpoint about DDR regulation, in which cytochrome c exerts a modulating role on such signalling cascade through its interaction with histone chaperones, both by tuning their nucleosome assembly and PP2A inhibitory activity.
Estudiantes de toda Europa explorarán cómo la innovación comparte procesos comunes en el arte y en la ciencia.
Dos investigadores del cicCartuja galardonados: Isabel Cruz-Gallardo, IBVF, y Juan C. Muñoz-García, IIQ.
Por primera vez se desarrolla un método sencillo y de bajo coste que une el compuesto metálico con esta especia
La Sociedad Española de Catálisis reconoce como mejor del 2014 a su trabajo doctoral realizado en el ICMS
El cicCartuja colabora en su organización para estrechar lazos entre el sector privado y los organismos públicos de investigación
The Academy acknowledges his studies of functional nanomaterials with applications in photonics, electronics and sensors