Título: Global identification of regulatory RNAs in the cyanobacterium Nostoc sp. PCC 7120. Functional characterization of Yfr1 and NsiR1
Doctorando: Francisco Rivero Rodríguez (IIQ)
Fecha: Viernes, 17 de enero de 2020. 11:00 h
Lugar: Salón de Actos
Directores de tesis: Dr. D. Miguel Ángel de la Rosa Acosta y la Dra. Dª. Irene Díaz Moreno
Resumen
The maintenance of genome integrity is a critical process for cell life. To face DNA lesions, living beings have developed a complex network called DNA damage response (DDR), which encompasses an intricate phosphorylation signalling cascade, enabling a quick DNA damage repair. To control this signalling cascade and to avoid an illegitimate activation, this network is tightly regulated by protein phosphatases, such as Protein Phosphatase 2A (PP2A). Moreover, histone chaperones assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several histone chaperones. Within this context, the role of nuclear cytochrome c has been partially disclosed.
Here, we have delved into the functional consequences of the novel DNA damage-induced complex formed by cytochrome c with the histone chaperones ANP32A and ANP32B. Strikingly, the heme protein impairs the PP2A inhibitory effect exerted by the histone chaperones ANP32A, ANP32B and SET/TAF-I. Furthermore, biophysical and structural approaches have been used to characterize the cytochrome c complex with ANP32A and ANP32B and to unveil the molecular mechanism underlying the cytoplasmic accumulation of ANP32B upon its phosphorylation.
In addition, we have extended our knowledge on the histone chaperone regulation in the DDR by studying the Arabidopsis thaliana complex between cytochrome c and NRP1, a SET/TAF-I analogous.
Herein we propose a novel standpoint about DDR regulation, in which cytochrome c exerts a modulating role on such signalling cascade through its interaction with histone chaperones, both by tuning their nucleosome assembly and PP2A inhibitory activity.
Miguel hizo su tesis en el MOM, el grupo de investigación del ICMS
Jesús es nombrado vicepresidente del Grupo Especializado de Resonancia Magnética Nuclear de la Real Sociedad Española de Química.
Los premiados en el concurso de 10alamenos9 en Andalucía, ya han recibido su reconocimiento y diploma acreditativo.
El investigador del ICMSE es editor jefe de esta prestigiosa revista, en la sección Nanomateriales
El investigador recibirá esta condecoración que reconoce la excelencia en la calidad científica y en la trayectoria profesional de jóvenes promesas españolas menores de 40 años.
Seleccionado en el XX Concurso Nacional para la adjudicación de Ayudas a la Investigación en ciencias de la Vida y de la Materia
Cristina, que hizo su doctorado en el ICMS, afrontará con su investigación el reciclaje de plásticos y residuos de biomasa para la generación de biopolímertos