< Alberto Jiménez Premio Extrardinario de Doctorado 2016-2017

New NMR methods for the study of carbohydrate conformation and protein carbohydrate interactions

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New NMR methods for the study of carbohydrate conformation and protein carbohydrate interactions. 

Dra. María Ángeles Canales Mayordomo (Universidad Complutense de Madrid).

Viernes 11 de octubre de 2019. 12:30 h.

Salón de Grados cicCartuja2

Abstract

Complex glycosylation patterns containing multiantennary N-glycans are typically found in mature glycoproteins. However,
the structural characterization of these glycans is rather challenging. Usually, standard NMR and X-ray diffraction techniques fail to provide specific answers on the structure and molecular recognition features due to intrinsic attributes
of the glycan. As a promising approach, carbohydrates conjugated to lanthanide binding tags have revealed high potential toward this aim. This methodology has first been applied to the study of small oligosaccharides (di-, tri- and tetrasaccharides). Proceeding from this experimental basis, we have extended this concept to the level of high degree branching and long chain N-glycans. In addition, the molecular recognition properties of these complex glycans were characterized in detailed thanks to the unprecedented resolution obtained in the spectra.

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