Título: Global identification of regulatory RNAs in the cyanobacterium Nostoc sp. PCC 7120. Functional characterization of Yfr1 and NsiR1
Doctorando: Francisco Rivero Rodríguez (IIQ)
Fecha: Viernes, 17 de enero de 2020. 11:00 h
Lugar: Salón de Actos
Directores de tesis: Dr. D. Miguel Ángel de la Rosa Acosta y la Dra. Dª. Irene Díaz Moreno
Resumen
The maintenance of genome integrity is a critical process for cell life. To face DNA lesions, living beings have developed a complex network called DNA damage response (DDR), which encompasses an intricate phosphorylation signalling cascade, enabling a quick DNA damage repair. To control this signalling cascade and to avoid an illegitimate activation, this network is tightly regulated by protein phosphatases, such as Protein Phosphatase 2A (PP2A). Moreover, histone chaperones assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell nucleus upon DNA damage, where it interacts with several histone chaperones. Within this context, the role of nuclear cytochrome c has been partially disclosed.
Here, we have delved into the functional consequences of the novel DNA damage-induced complex formed by cytochrome c with the histone chaperones ANP32A and ANP32B. Strikingly, the heme protein impairs the PP2A inhibitory effect exerted by the histone chaperones ANP32A, ANP32B and SET/TAF-I. Furthermore, biophysical and structural approaches have been used to characterize the cytochrome c complex with ANP32A and ANP32B and to unveil the molecular mechanism underlying the cytoplasmic accumulation of ANP32B upon its phosphorylation.
In addition, we have extended our knowledge on the histone chaperone regulation in the DDR by studying the Arabidopsis thaliana complex between cytochrome c and NRP1, a SET/TAF-I analogous.
Herein we propose a novel standpoint about DDR regulation, in which cytochrome c exerts a modulating role on such signalling cascade through its interaction with histone chaperones, both by tuning their nucleosome assembly and PP2A inhibitory activity.
El Premio Reaxys RSEQ auspiciado por Elsevier y la Real Sociedad Española de Química reconoce su valor como joven investigador a nivel nacional
Investigadores del ICMS realizan charlas, talleres y encuentros en las bibliotecas de San Jerónimo y de Montequinto (Dos Hermanas)
La actividad, en la que los alumnos premiados en la XV Feria de la Ciencia presentarán sus proyectos, tendrá lugar el 16 de noviembre
En esta edición nos visitarán alumnos de bachillerato de las Escuelas Francesas que participarán en tres mesas-coloquios el próximo día 14 de noviembre
El premio valorado en 1000 € lo otorga el Grupo Especializado de Calorimetría y Análisis Térmico de la RSEQ y la RSEF
El investigador del IBVF ha sido nombrado miembro del comité conjunto FEPS-ISOP en representación de la Federación Europea de Sociedades de Protistología