cicCartuja Centro de Investigaciones Científicas de la cartuja

Cysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in Arabidopsis

IBVF

Consolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia Gotor The Plant Cell 2013, Vol. 0, DOI: 10.1105/tpc.112.105403

In Arabidopsis thaliana, DES1 is the only identified L-Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine (Cys) and the concomitant production of H2S in this cell compartment. Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided insight into the role of sulfide metabolically generated in the cytosol as a signaling molecule. Mutations of L-CYS DESULFHYDRASE 1 (DES1) impede H2S generation in the Arabidopsis cytosol and strongly affect plant metabolism. Senescence-associated vacuoles are detected in mesophyll protoplasts of des1mutants. Additionally, DES1 deficiency promotes the accumulation and lipidation of the ATG8 protein, which is associated with the process of autophagy. The transcriptional profile of the des1-1 mutant corresponds to its premature senescence and autophagy-induction phenotypes, and restoring H2S generation has been shown to eliminate the phenotypic defects of des1 mutants. Moreover, sulfide is able to reverse ATG8 accumulation and lipidation, even in wild-type plants when autophagy is induced by carbon starvation, suggesting a general effect of sulfide on autophagy regulation that is unrelated to sulfur or nitrogen limitation stress. Our results suggest that Cys-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile of Arabidopsis.

• Effect of gold on a NiLaO3 perovskite catalyst for methane steam reforming ICMS Sandra Palma et al.
• Implementing the “Catch-and-Release” Concept... IIQ Iris Pflueger et al.
• Resonant Photocurrent Generation in Dye-Sensitized... ICMS Miguel Anaya et al.
• Building a Parent Iridabenzene Structure from Acetylene... IIQ Vivancos A. et al.
• Loss of starch granule initiation effect on Arabidopsis thaliana growth IBVF Ragel P et. al.
• Dibenzyl and diallyl 2,6-bisiminopyridinezinc (II) complexes: selective... IIQ Sandoval et. al.
• Angular response of photonic crystal based dye sensitized solar cells ICMS López-López et. al.
• Glyconanosomes: Disk-Shaped Nanomaterials for the Water Solubilization... IIQ Mohyeddin Assali et al.
• Interconversion of Quadruply and Quintuply Bonded Molybdenum... IIQ Mario Carrasco et al.
• Selective UV Reflecting Mirrors Based on Nanoparticle Multilayers ICMS José R. Castro Smirnov et al.
• Virus-like glycodendrinanoparticles displaying... IIQ Renato Ribeiro-Viana et al.
• Cysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy... IBVF Consolación Álvarez et al.